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Anth 125 Introduction to Biological Anthropology
Dr. Darlene Applegate
Spring 2008
GROUP LAB PROJECT 4:
PHENOTYPIC VARIATION IN SIMPLE TRAITS




INTRODUCTION

Modern human biological variation is the study of genotypic and phenotypic variation among contemporary humans. The questions of interest are how humans vary and why humans vary, and these questions are investigated using an evolutionary approach. In this lab we examine phenotypic variation in ten simple traits among members of the class.


OBJECTIVES

TERMS

SIMPLE TRAITS

Simple traits are those that are coded by alleles at one gene locus. They are also called monogenic, discrete, or qualitative traits. Some simple traits – such as white hair forelock – are coded by two alleles, while other simple traits – such as ABO blood group – are coded by three alleles. Simple traits are determined largely by one’s genetic makeup (i.e., the alleles one inherits) as opposed to the environment. There are thousands of simple traits in humans. For this group project, we will examine phenotypic expressions of the following ten simple traits (France 2004).

PTC Tasting:  Phenylthiocarbamide (PTC) is human-made chemical that is similar to a naturally occurring substance found in turnip greens, brussel sprouts, kale, and other leafy green vegetables. People who can taste this chemical are likely to find the aforementioned plants bitter-tasting and, therefore, unlikely to eat large quantities of these plants. Consumption of large quantities of these plants can increase an individual’s likelihood of suffering from goiter, which is an enlargement of the thyroid. Individuals with at least one dominant allele are able to taste PTC. Since we do not have PTC samples to taste, record your phenotype as taster/dominant if you think turnip greens, brussel sprouts, kale, and mustard greens taste bitter.

Tongue Rolling:  Tongue rolling refers to the ability to roll the sides of one’s tongue upward to create a tube-like shape. Individuals who can roll their tongues have at least one dominant allele for the trait.

Tongue Folding: Tongue folding refers to the ability to fold the tip of one’s tongue backward. Individuals who can fold their tongues have two recessive alleles for the trait.

Earlobe Attachment:  Earlobes are either free-hanging, meaning that the lower boundary of the earlobe dangles away from the neck, or attached, meaning that the lower boundary of the earlobe is connected directly to the neck. Individuals with free-hanging earlobes have at least one dominant allele, while individuals with attached earlobes have two dominant alleles for the trait.

ear freeear attach
Free-hanging (left) and attached (right) ear lobes.
Images from http://www.jbhs.k12.nf.ca/biology/EARS.HTM


Darwin’s Tubercle:  Darwin’s tubercle is a thickening of the cartilage of the upper-inner rim of the ear. It may be present on one or both ears. Individuals with at least one Darwin’s tubercle have at least one dominant allele for the trait.

tubercle
Darwin's tubercle.
Image from http://pinna.hawkelibrary.com

Hitchhiker’s Thumb:  The ability to bend the upper joint of the thumb backward more than 50 degrees is referred to as hitchhiker’s thumb. Individuals who have this ability have two recessive alleles for the trait.

thumb
Hitchhiker's thumb. Image from http://www.visualsunlimited.com

Hand Folding:  When one folds his/her two hands together such that the fingers interlock, there is a natural tendency for either the left thumb or the right thumb to be placed on top. Individuals who place the left thumb on top have at least one dominant allele, while individuals who place the right thumb on top have two recessive alleles.

Hair Whorl:  The hair on the top of an individual’s head either whorls in a clockwise (CW) direction or in a counterclockwise (CCW) direction. The former condition is inherited as a dominant, while the latter condition is inherited as a recessive.

Widow’s Peak:  The hairline above the forehead may be roughly horizontal or it may point in a V-shape. The latter phenotype is referred to as widow’s peak and is inherited as a recessive.

Ear Wax:  There are two forms of ear wax in humans. Sticky, yellow ear wax is inherited as a dominant, while dry, gray ear wax is inherited as a recessive.


ASSIGNMENT


Each student will bring a calculator and a pencil to the class session. Students will work in groups of three to complete the project. Each group will submit one group answer sheet.

Simple Trait Inventory

The first task is to record each group member’s phenotypes for the ten simple traits. Record this data by circling the appropriate phenotype for each trait for each group member on the first page of the answer sheet.

These data will be entered by the instructor into an Excel spreadsheet and displayed using the Proxima LCD projector. Each group will use this spreadsheet to evaluate phenotypic variation, as described below.

Phenotypic Variation

The second task involves calculating sample sizes and percentages for each phenotype for each of the ten simple traits. We will do so for the entire class (total sample), for males only, and for females only.

First, record the sample sizes for the entire class, males only, and females only. (The number of males plus females must equal the total sample size.)

Second, record the total number of individuals exhibiting the dominant phenotype for PTC tasting and convert this into a percentage. Do the same for the recessive phenotype for PTC tasting for the total sample. (To calculate a percentage, divide the number of individuals with the dominant phenotype by the sample size and then multiply that answer by 100. Unless the answer is a whole number, all percentages must be rounded to tenths place.) Repeat for males only and for females only for the PTC trait. Then repeat for the other nine simple traits.

Third, for each trait indicate which phenotype is the most common among the class members.

Fourth, compare the male and female percentages for each trait and indicate which traits exhibit significant phenotypic variation between the sexes. (If the difference between male and female phenotype percentages is greater than 20.0%, consider that a significant difference.)


REFERENCE


France, Diane
2004  Lab Manual and Workbook for Physical Anthropology (5th edition). Thomson Wadsworth, Belmont, CA.



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